Biological Engineering (BE) lies at the interface between biology and engineering and aims at designing and constructing biological parts or biological systems. BE relies on a forward-design approach using the key concepts of modularity, insulation, standardization and scalability. This constructive approach allows to elucidate the fundamental principles of biological system organization, filling the gap between description and understanding of biological systems. On the other hand, BE constructive approach is used to develop effective biology-based technologies for applications across a wide spectrum of societal needs including: innovative medicines (red biotechnology), generate new sources of energy and chemical processes (white biotechnology) and develop novel application in the environment-agrofood sector (green biotechnology). At ECLT we pursue the design, synthesis and standardization of completely de novo proteins to be exploited as novel functional scaffolds for BE. As a prerogative, our interdisciplinary approach integrates molecular biology, complex system science and advanced statistical methodologies to harness the full potential of synthetic biology.